玉米FBA基因家族成员鉴定及表达分析

Genome-wide Identification and Expression Analysis of FBA gene family in Maize

摘要:

为了解玉米ZmFBAs家族成员的基本信息，以8个拟南芥AtFBAs家族成员蛋白序列为基础，通过本地Blastp对玉米全基因组进行同源序列比对，并对获得的ZmFBAs进行系统的生物信息学和表达谱分析。结果表明，鉴定到7个玉米ZmFBAs基因家族成员，含TIM superfamily和PLN02425两种典型保守结构域。根据进化关系和保守结构域，ZmFBAs可分为两个亚家族，分别对应亚细胞定位预测的质体和胞质，ZmFBAs启动子区富含光响应、激素响应、逆境响应等顺式作用元件。玉米ZmFBAs分布在5条染色体上，其中两对基因发生了片段复制事件。ZmFBAs在不同组织中呈现多种表达模式，具有组织表达特异性，ZmFBA4、ZmFBA6和ZmFBA7在所有组织中均表达，ZmFBA3为不表达基因。ZmFBAs在响应生物和非生物胁迫时呈现出不同的表达模式。

Abstract:

To investigate the basic information of ZmFBA family in maize, ZmFBA members were identified in the whole genome of maize inbred B73 by alignment with eight Arabidopsis thaliana AtFBA genes, and then systematically analyzed them by bioinformatics and expression profile. The results showed that seven ZmFBA family members were identified, which contain TIM superfamily and PLN02425 conserved domain. Based on evolutionary relationships and conserved domains, ZmFBAs can be divided into two subfamilies corresponding to chloroplasts aldolase and cytoplasms aldolase predicted by subcellular localization. The promoter regions of ZmFBAs harbored multiple cis-elements related to light response, plant hormone response, environmental stresses, and other situations. Seven ZmFBA family members distributed on five chromosomes, respectively, two segment duplicated gene pairs of ZmFBA family in maize. The ZmFBA family genes exhibited multiple expression patterns in different tissues, showing strong tissues-specific expression. Among them ZmFBA4, ZmFBA6, ZmFBA7 expressed in all tissues, and ZmFBA3 was not expressed under the current experimental conditions. ZmFBAs responded to various biological and abiotic stresses, exhibited multiple expression patterns.